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sw 48  (ATCC)


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    Structured Review

    ATCC sw 48
    MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and <t>SW-48.</t> ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.
    Sw 48, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Exceptionally selective voltage-sensor trapping of Na V 1.5 channels by Mg-protoporphyrin impairs cancer cell migration"

    Article Title: Exceptionally selective voltage-sensor trapping of Na V 1.5 channels by Mg-protoporphyrin impairs cancer cell migration

    Journal: Scientific Reports

    doi: 10.1038/s41598-026-37492-0

    MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and SW-48. ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.
    Figure Legend Snippet: MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and SW-48. ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.

    Techniques Used: Migration, Expressing, Control, Staining, Transwell Migration Assay, Transmission Assay, Cell Counting, Software, Wound Healing Assay



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    MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and <t>SW-48.</t> ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.
    Sw 48, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    human colon cells sw 48 cell lines  (ATCC)
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    MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and <t>SW-48.</t> ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.
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    sw 48 cells  (ATCC)
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    MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and <t>SW-48.</t> ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.
    Sw 48 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and <t>SW-48.</t> ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.
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    MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and <t>SW-48.</t> ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.
    Sw 48 Ccl 231 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and SW-48. ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.

    Journal: Scientific Reports

    Article Title: Exceptionally selective voltage-sensor trapping of Na V 1.5 channels by Mg-protoporphyrin impairs cancer cell migration

    doi: 10.1038/s41598-026-37492-0

    Figure Lengend Snippet: MgPpIX slows down the migration of cancer cells expressing SCN5A. ( A ) Representative images of MDA-MB-231 cell cultures right after introducing a scratch gap (0 h) and 4 h later. After scratch introduction, the cells were kept in control medium with DMSO (Ctrl) or 100 nM of the indicated MePpIX, dissolved in DMSO. The cells were kept in the dark to prevent phototoxicity induced by the MePpIXs. Continuous black lines indicate the automatically detected scratch borders. Scale bar: 400 µm. ( B ) Mean gap-closure migration of MDA-MB-231 cells (relative to the migration under control conditions, i.e., in the presence of the vehicle DMSO) in the presence of 100 nM of PpIX or the indicated MePpIX variants. C Representative confocal images of DAPI-stained MDA-MB-231 cell nuclei detected on the trans side in a transwell migration assay with an 8 µm pore diameter and a chemoattraction gradient from 0 (insert) to 2% FBS (wells); background in white. The panels on the right show superpositions of the DAPI staining (blue) with transmission images. Cells were kept overnight under control conditions or in the presence of 100 nM of the indicated MePpIX. Images were taken through a 10 × objective with a 3 × zoom factor. Cell counting was performed based on the DAPI staining using ImageJ software. Scale bar: 400 µm. ( D ) Mean number of migrated cells from experiments as shown in ( C ). E Mean migration in a wound-healing assay of MDA-MB-231 cells over 4 h in the presence of the indicated concentrations of MgPpIX, TTX, and PpIX. ( F ) Mean normalized current traces obtained from MDA-MB-231 cells under voltage-clamp conditions with step depolarizations to − 30 mV without (black) and with the application of 100 nM MgPpIX. The currents on the right followed an episode of 250 ms at 40 mV to highlight the reverse use-dependence of these Na V channels. ( G ) Mean relative migration in a wound-healing assay (as in B and E ) but for T-47D cells. ( H ) As in ( G ) but for the colon carcinoma cell lines SW-480 and SW-48. ( I ) Mean current density, based on the peak currents at − 30 mV, for HEK293T cells and the indicated breast cancer (MDA-MB-231, T-47D) and colon carcinoma cells (SW-480, SW-48). Data bars are means ± sem with n in parentheses; in ( B ), ( D ), ( E ), ( G ), and ( H ), the number of independent cell batches is given.

    Article Snippet: The breast cancer cell lines MDA-MB-231 (DSMZ) and T-47D (American Type Culture Collection, ATCC) were cultured in RPMI 1640 medium (Sigma, R7388), while the colorectal cancer cell lines SW-480 (DSMZ) and SW-48 (ATCC) were maintained in DMEM.

    Techniques: Migration, Expressing, Control, Staining, Transwell Migration Assay, Transmission Assay, Cell Counting, Software, Wound Healing Assay